Journal: Scientific Reports

Article Title: Tai Chi Chuan vs General Aerobic Exercise in Brain Plasticity: A Multimodal MRI Study

doi: 10.1038/s41598-019-53731-z

Figure Lengend Snippet: FC results. The results of resting-state functional connectivity analysis are presented on axial slices of the grey matter template (MNI). Yellow and red indicate brain regions that showed significant FC increase (post > pre) in the Tai Chi Chuan group. The threshold for significant changes was set to p < 0.05 cluster mass-level FWE corrected with a cluster building threshold of p = 0.001 uncorrected on voxel level.

Article Snippet: Thirty-six college students were grouped into Tai Chi Chuan (Bafa Wubu of Tai Chi), general aerobic exercise (brisk walking) and control groups.

Techniques: Functional Assay

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: Cells were exposed with 3-MA (10 mM) and Baf.A (1 nM) alone or with gefitinb (5 μM) for 48 hours, and the inhibitory were confirmed by immunofluorescence staining. LC3 puncta in the cells were detected by immunofluorescence under confocal laser microscopy in MDA-MB-231 (A) and MDA-MB-468 (B).

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Immunofluorescence, Staining, Microscopy

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: (A) MDA-MB-231 and MDA-MB-468 cells were treated with 0, 1.25, 2.50, 5.00, 10.00, 20.00 μM Ge alone or combined with 3-MA (10 mM) or Baf.A (1 nM) respectively for 48 hours, DMSO acted as the control, and then subjected to CCK8 assay. Absorbance value was calculated and standardized to DMSO group. Three independent experiments were performed. (B) The above cells were treated with DMSO (0.2%), 3-MA (10 mM), Baf.A (1 nM), Ge (5 μM), Ge (5 μM) +3-MA (10 mM) and Ge (5 μM) +Baf.A (1 nM), DMSO acted as the control, and subjected to cell colony formation assay. (C) Cell surviving fraction were calculated and presented as mean ± SD; *p < 0.05. Three independent experiments were performed.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: CCK-8 Assay, Colony Assay

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: MDA-MB-468 xenograft tumor was established and treated as follows: DMSO, 3-MA (1.0 mg/kg), Baf.A (1.0 mg/kg), Ge (100 mg/kg), Ge (100 mg/kg) +3-MA (1.0 mg/kg) and Ge (100 mg/kg) +Baf.A (1.0 mg/kg). 3-MA and Baf.A were injected intratumorally, Ge was administered via oral gavage. Tumor growth curves (A) , tumor weight (B) , tumor image (C) , and nude mouse image (D) with different treatment were detected. The results are shown as means ± SD; *p < 0.05.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Injection

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. The cycle distributions of MDA-MB-231 and MDA-MB-468 cells were analyzed.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques:

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: Cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours. Phospho-ATM, Phospho-Chk1, Phospho-Chk2, γ-H2AX, and ACTB of MDA-MB-231 and MDA-MB-468 cells were analyzed by western blot.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Autophagy inhibitor facilitates gefitinib sensitivity in vitro and in vivo by activating mitochondrial apoptosis in triple negative breast cancer

doi: 10.1371/journal.pone.0177694

Figure Lengend Snippet: The above cells were treated with DMSO, 3-MA, Baf.A, Ge, Ge+3-MA and Ge+Baf.A for 48 hours and were subjected to western blot using following antibodies, Cytochrome C (Cyto C), cleaved CASP3, BAX, Bcl-2 and ACTB (A) . Hematoxylin-eosin staining (B) and immunohistochemical assays (anti-cleaved CASP 3) (C) were performed in MDA-MB-468 xenograft.

Article Snippet: Ge, 3-MA and Baf.A were dissolved in 100% dimethyl sulfoxide (DMSO; Fisher Scientific, Pittsburgh, PA, USA).

Techniques: Western Blot, Staining, Immunohistochemical staining